General information
SALSA
® MLPA
® Probemix P175 Tumour Gain is a
research use only (RUO) assay for the detection of copy number aberrations in 24 genes, which are frequently gained or amplified in various tumour types. This probemix can also be used to detect the presence of the
BRAF p.V600E (c.1799T>A) point mutation.
This product is not CE/FDA registered for use in diagnostic procedures. The SALSA® MLPA® technique is covered by US patent 6,955,901 and corresponding patents outside the US. The purchase of this product includes a license to use only this amount of product solely for the purchaser’s own use.
Probemix content
P175-B2 Tumour Gain contains 62 MLPA probes with amplification products between 115 and 504 nucleotides (nt). This includes two probes for each of the following genes:
ABL1,
ALK,
AR, AURKA/B,
BRAF,
CCND1/2,
CDK4,
DHFR,
EGFR,
ERBB2,
FGFR1,
KDR,
KIT,
MDM2/4,
MET,
MYC,
MYCN,
PDGFRA,
RET,
SMO and
TOP2A. Furthermore, this probemix also contains one probe specific for the
BRAF p.V600E (c.1799T>A) point mutation which will only generate a signal when the mutation is present. In addition, 13 reference probes are included that target relatively copy number stable regions in various cancer types. Partial probe sequences are available in Table 2 and 3, and online (
www.mrcholland.com). The identity of the genes detected by the reference probes is available in Table 3.
This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at
www.mrcholland.com.
SALSA® Binning DNA SD029
The SALSA
® Binning DNA SD029 provided with this probemix can be used for binning of all probes including one mutation-specific probe (
BRAF p.V600E (c.1799T>A) probe 08780-SP0039-L08904). SD029 is a mixture of human female genomic DNA from healthy individuals and a titrated amount of plasmid DNA that contains the target sequence detected by the above mentioned probe. Inclusion of one reaction with 5 μl SD029 in initial MLPA experiments is essential as it can be used to aid in data binning of the peak pattern using Coffalyser.Net. Furthermore, binning DNA should be included in the experiment whenever changes have been applied to the set-up of the capillary electrophoresis device (e.g. when capillaries have been renewed). Binning DNA should never be used as a reference sample in the MLPA data analysis, neither should it be used in quantification of mutation signal. For further details, please consult the SD029 product description, available online:
www.mrcholland.com.
This product is for research use only (RUO).
Sample DNA
Sample DNA developed for this product: