General information
The SALSA MLPA
Probemix P181 Centromere mix 1 is a
research use only (RUO) assay for the detection of deletions or duplications in genes close to the centromeres of all chromosomes, with the exception of the Y-chromosome. In most cases, probes are included for the first well-characterised gene in the centromeric region. Possible applications of this probemix are in cancer research, as well as for characterisation of marker chromosomes and the detection of aneuploidies.
This SALSA MLPA probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.
Probemix content
The SALSA MLPA Probemix P181-C1 Centromere mix 1 contains 46 MLPA probes with amplification products between 127 and 450 nucleotides (nt). This includes one probe for each of the chromosome arms (except the Y-chromosome). For the acrocentric chromosomes (13, 14, 15, 21 and 22), which have more than 10 Mb of repeat sequences at one end covering most or all of the p-arms, there are two probes on the q-arm, close to the centromere. The SALSA MLPA Probemix P182 Centromere mix 2 detects different sequences in the same regions. Complete probe sequences are available online (
www.mrcholland.com).
This probemix contains ten quality control fragments generating amplification products between 64 and 121 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and two chromosome Y-specific fragments. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at
www.mrcholland.com.