General information
The SALSA MLPA
Probemix P297 Microdeletion-2 is a
research use only (RUO) assay for the detection of deletions or duplications in the following chromosomal regions:
• 1q21.1 (TAR) 5 probes
• 1q21.1 (distal) 6 probes
• 3q29 4 probes
• 15q13 10 probes
• 15q24 4 probes
• 16p13.11 4 probes
• 16p12.1 3 probes
• 16p12.1-p11.2 3 probes
• 16p11.2 (distal) 3 probes
• 16p11.2 (proximal) 4 probes
• 17q12 8 probes
More information about these Microdeletion Syndromes is available below Table 2.
This SALSA MLPA probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.
Probemix content
The SALSA MLPA Probemix P297-D1 Microdeletion-2 contains 54 MLPA probes with amplification products between 115 and 519 nucleotides (nt). This includes 54 probes for the chromosomal regions 1q21.1, 3q29, 15q13, 15q24, 16p13.11, 16p12.1-p11.2, and 17q12. Complete probe sequences are available online (
www.mrcholland.com).
This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at
www.mrcholland.com.