General information
SALSA
® MLPA
® Probemix P303 Medulloblastoma mix 3 is a
research use only (RUO) assay for the detection of deletions or gains in chromosomes 1, 4q, 5q, 8, 10 and 20, which are suggested to be associated with medulloblastoma.
Medulloblastoma (MB) is the most common paediatric primary central nervous system (CNS) cancer type, accounting for 15% to 20% of CNS tumours in patients under the age of 20. It is a highly invasive embryonal neuroepithelial tumour that arises in the cerebellum and has a tendency to disseminate throughout the CNS early in its course. Overall survival is 50-60% at five years, although this decreases to 30% in the longer term due to local recurrence and/or metastasis. There are four distinct molecular subtypes of MB (WNT, sonic hedgehog (SHH), Group 3, and Group 4) which can be used for patient risk stratification and that have the potential to guide new therapeutic strategies for the treatment of MB (Taylor et al. 2012). These molecular subtypes of MB include characteristic and recurrent copy number alterations, which are covered by the P301, P302 and P303 Medulloblastoma probemixes.
This product is not CE/FDA registered for use in diagnostic procedures. The SALSA® MLPA® technique is covered by US patent 6,955,901 and corresponding patents outside the US. The purchase of this product includes a license to use only this amount of product solely for the purchaser’s own use.
Probemix content
P303-A3 Medulloblastoma mix 3 contains 43 MLPA probes with amplification products between 121 and 495 nucleotides (nt). This includes 31 probes for chromosomes 1, 4q, 5q, 8, 10 and 20. In addition, 12 reference probes are included that target relatively copy number stable regions in various cancer types, including medulloblastoma. Complete probe sequences and the identity of the genes detected by the reference probes are available online (
www.mrcholland.com).
This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at
www.mrcholland.com.