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SALSA MLPA Probemix P451 Chromosome 16

Breast cancer + various tumour types

Region: Chromosome 16

General information
The SALSA MLPA Probemix P451 Chromosome 16 is a research use only (RUO) assay for the detection of deletions or duplications on chromosome 16, which are associated with several tumour types, including breast cancer, Wilms’ tumour and multiple myeloma.

Low-grade breast neoplasias are molecularly characterized by a loss of chromosome arm 16q (De Boer et al. 2018; Rakha et al. 2006). Deletions of 16q arm are amongst the most frequent genetic events in breast cancer, especially in low grade ductal carcinoma, and loss of 16q seems to be associated with better prognosis. Since a loss of chromosome 16q has also been detected in premalignant lesions, this loss is likely to encompass an early step in carcinogenesis (De Boer et al. 2018). Loss of 16q is associated with poor prognosis in multiple myeloma (Jenner et al. 2006) and loss of heterozygosity for 16q is a prognostic factor identifying a high risk of relapse and death in Wilms’ tumour (Grundy et al. 2005).

Probemix content
The SALSA MLPA Probemix P451-B1 Chromosome 16 contains 50 MLPA probes with amplification products between 130 and 500 nucleotides (nt). This includes 36 probe(s) for chromosome 16, targeting 35 genes. In addition, 14 reference probes are included that target relatively copy number stable regions in various cancer types, including breast cancer. Probe sequences and the identity of the genes detected by the reference probes are available in Table 3 of the product description and online (www.mrcholland.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment (see table below). More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mrcholland.com.

Order Items

Probemix

Item no.
Description
Technology
Price
P451-025R
SALSA MLPA Probemix P451 Chromosome 16 – 25 rxn
€ 281.00
P451-050R
SALSA MLPA Probemix P451 Chromosome 16 – 50 rxn
€ 550.00
P451-100R
SALSA MLPA Probemix P451 Chromosome 16 – 100 rxn
€ 1075.00

Required Reagents (Sold Separately)

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
€ 341.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
€ 341.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
€ 1571.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
€ 1571.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
€ 6037.00

Related Products

SALSA MLPA Probemix P078 Breast tumour

Contains probes for CDH1, as well as eight other chromosomal regions relevant for breast cancer.

SALSA MLPA Probemix P380 Wilms' tumour

Contains probes for 16p and 16q, as well as two other chromosomal regions and five genes relevant for Wilms’ tumour.

SALSA MLPA Probemix P425 Multiple Myeloma

Contains probes for 16q12 and 16q23, as well as nine other chromosomal regions relevant for multiple myeloma.

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