General information
The SALSA MS-MLPA
Probemix ME001 Tumour suppressor mix is a
research use only (RUO) assay for the detection of aberrant methylation of one or more sequences of 25 tumour suppressor genes (TSG). This probemix can also be used to detect deletions/duplications in these TSGs in a DNA sample.
CpG-islands are located in or near the promoter region or other regulatory regions of approximately 50% of human genes. Aberrant methylation of CpG-islands has been shown to be associated with transcriptional inactivation of genes in a wide spectrum of human cancers. These TSGs are frequently silenced by methylation in tumours, but are unmethylated in blood-derived DNA. In addition, DNA methylation analysis can indicate in some cases from which type of tissue the tumour was derived.
This SALSA MS-MLPA probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.
Probemix content
The SALSA MS-MLPA Probemix ME001-D1 Tumour suppressor mix contains 45 (MS-)MLPA probes with amplification products between 121 and 495 nucleotides (nt). 28 MS-MLPA probes contain an HhaI recognition site and provide information on the methylation status of 25 TSGs. All probes present will also give information on copy number changes in the analysed sample. In addition, 16 reference probes are included that are not affected by HhaI digestion and target relatively copy number stable regions in various cancer types. Also, one digestion control probe is included in this probemix indicating whether or not restriction endonuclease digestion in the MS-MLPA reaction was complete. Complete/partial probe sequences and the identity of the genes detected by the reference probes are available in Tables 2 and 3 of the Product Description, and online (
www.mrcholland.com).
This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MS-MLPA General Protocol and online at
www.mrcholland.com.